ETAPE (engleza)
SCIENTIFIC REPORT - STAGE I
Stage Number/Stage Name:
Stage I: Reconfiguration of the detection setup for precise and specific measurements of trace gases (NH3, C2H4, and CO2).
Stage I Objectives:
Activity 1.1: Determination of detection limits for each gas component.
Activity 1.2: Development of multi-gas detection analysis by designing new software programs for both data acquisition and processing.
Planned Results for Stage I:
The scientific report describes the experimental model along with parameters and specific characteristics of the photoacoustic system for optimal determination of exhaled biomarkers in patients with H. pylori.
Participation in a scientific conference; Research progress will be reported online and regularly updated using the INFLPR website, LinkedIn, and Facebook.
Results Achieved at the End of Stage I:
Scientific report describing the experimental model along with parameters and specific characteristics of the photoacoustic system for optimal determination of exhaled biomarkers in patients with H. pylori.
Publication of one article in an ISI-rated journal, 7 participations in scientific conferences; creation/update of both the project website and Facebook page.
Executive Summary of Activities During the Implementation Period:
The first stage, focusing on the reconfiguration of the detection setup for precise measurements of trace gases (NH3, C2H4, and CO2), is dedicated to adjusting and optimizing the detection setup for the monitored analysis of multiple gases in human breath. This platform for multi-gas detection is suitable for monitoring NH3, C2H4, and CO2 in the exhaled breath of patients infected with Helicobacter pylori.
The first stage includes:
Determination of detection limits for each gas component: Reconfiguration of the current experimental setup based on new experimental conditions to perform appropriate measurements. Testing the new setup configuration to obtain optimal results in gas detection.
Development of multi-gas detection analysis by designing new software programs for both data acquisition and processing: Calibration of the photoacoustic cell for the gases of interest (NH3, C2H4, and CO2), considering both single-component and multi-component gas mixtures, to verify theoretical results, achieve detection limits for each individual gas component, and use them in subsequent determinations. This step will be performed through the design of dedicated software programs for both data acquisition and data processing.
SCIENTIFIC REPORT - STAGE II
Stage Number/Stage Name: Testing the breath analysis setup on patients with Helicobacter pylori.
Stage II Objectives:
Activity 2.1: Preliminary results of measurements of ammonia and CO2 in the breath of patients with Helicobacter pylori.
Activity 2.2: Preliminary results of oxidative damage assessment and correlation of the ethylene biomarker with immune dysfunction in patients with Helicobacter pylori.
Planned Results for Stage II:
Scientific report on the concentration of ammonia, carbon dioxide, and ethylene in the case of H. pylori infection.
Participation in a scientific conference; Project website update, LinkedIn, Facebook; 1 scientific article in an ISI-rated journal.
Results Achieved at the End of Stage II:
Scientific report with preliminary results regarding the concentration of ammonia, carbon dioxide, and ethylene in subjects with Helicobacter pylori.
Publication of 4 articles (2 articles in ISI-rated journals and 2 popular science articles), and submission of 1 article for evaluation in an ISI-rated journal; 10 presentations at scientific conferences; creation/update of both the project website and Facebook page.
Executive Summary of Activities During the Implementation Period:
The second stage, dedicated to testing the breath analysis setup on patients with Helicobacter pylori, focuses on monitoring NH3, C2H4, and CO2 levels in the exhaled breath of subjects infected with Helicobacter pylori and healthy subjects.
This stage includes:
Activity 2.1: Preliminary results of measurements of ammonia and CO2 in the breath of patients with Helicobacter pylori: This activity describes the protocol with new experimental conditions for performing appropriate measurements and analysis of ammonia and CO2 concentrations in human breath.
Activity 2.2: Preliminary results of oxidative damage assessment and correlation of the ethylene biomarker with immune dysfunction in patients with Helicobacter pylori: This activity describes the protocol with new experimental conditions for performing appropriate measurements and analysis of ethylene concentrations in human breath.
SCIENTIFIC REPORT - STAGE III
Stage Number/Stage Name: Validation of the detection platform capable of measuring multiple gas concentrations in Helicobacter pylori patients and Management/dissemination of the results: (Project management, Strategic coordination, Intellectual property rights - IPR management, Dissemination activities.)
Stage III Objectives:
Activity 3.1: Characteristics of the detection platform after optimization.
Activity 3.2: Statistical evaluation of multiple gases from patients.
Planned Results for Stage III:
Final technical report for the experimental model and Scientific Report with the statistics of the concentration of ammonia, ethylene and carbon dioxide in the human breath of subjects with Helicobacter pylori infection, Updating the project website , 1 participation at the international conference, 1 patent application submission; 2 articles in ISI journals.
Results Achieved at the End of Stage III:
4 articles in ISI journals, 3 articles in non- ISI journals, 3 participations at the international conferences, 1 patent application submission and Final technical report for the experimental model with Scientific Report with the statistics of the concentration of ammonia, ethylene and carbon dioxide in the human breath of subjects with Helicobacter pylori infection.
Executive Summary of Activities During the Implementation Period:
The third stage, dedicated to Validating the detection platfrom capable of measuring multiple gas concentrations in Helicobacter pylori subjects and Management/dissemination of the results: (Project management, Strategic coordination, Intellectual property rights - IPR management, Dissemination activities).
This stage includes:
Activity 3.1: Characteristics of the detection platform after optimization: This activity presents both the main characteristics of the experimental setup for monitoring trace gases and the measurable factors for the detection of gases from human breath present in the breath of subjects with Helicobacter pylori and the breath of healthy volunteers. In this activity also the noises and the limiting factors in the photoacoustic spectroscopy were studied.
Activity 3.2: Statistical evaluation of multiple gases from patients: In this activity, multiple gases from human breath were evaluated using the statistical analysis program GraphPad Prism.